hTNFRSF12A

Fig.1 Detection of TNFRSF12A expression in adrenal and ovary by RT-PCR. Tissues RNA was extracted from a 11-week-old female WT C57BL/6 mosue and 8-week-old female homozygous hTNFRSF12A knockin mice (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse Tnfrsf12a mRNA (135 bp) was detectable only in wild-type C57BL/6 mice. Human TNFRSF12A mRNA (104 bp) was detectable only in homozygous hTNFRSF12A knockin mice but not in wild-type mice. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of TNFRSF12A expression in hTNFRSF12A mice by WB. The heart, kidney and skeletal muscle tissue lysates were collected from 11-week-old female WT C57BL/6 mice and 8-week-old female homozygous hTNFRSF12A mice, and then analyzed by western blot with anti-TNFRSF12A antibody. Human TNFRSF12A protein was detectable in heart, kidney and skeletal muscle tissues from hTNFRSF12A knockin mice using an antibody cross-reactive between human and mouse TNFRSF12A.

Abbr. HO, homozygous; WT, wild type; PC, positive control, HT-1080 cells; M, marker.

Note. The arrow indicates the target strip.