hCCN4

Fig.1 Detection of CCN4 expression in ovary by RT-PCR. Ovary RNA was extracted from 6-week-old female wild-type C57BL/6 (WT)(n=2) and homozygous hCCN4 knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse Ccn4 mRNA (262 bp) was detectable only in wild-type C57BL/6 mice. Human CCN4 mRNA (265 bp) was detectable only in homozygous hCCN4 knockin mice but not in wild-type mice. Mouse Ccn4 mRNA (262 bp) was detectable only in wild-type C57BL/6 mice. Human CCN4 mRNA (265 bp) was detectable only in homozygous hCCN4 knockin mice but not in wild-type mice. 

Fig.2 Detection of CCN4 expression in hCCN4 mice by WB. Lysates of heart, liver, spleen, lung and kidney tissues were collected from 6-week-old female wild-type C57BL/6 mice and 6-week-old female homozygous hCCN4 knockin mice, and then analyzed by western blot with anti-CCN4 antibody. CCN4 was detectable in heart, liver, spleen, lung and kidney from both WT C57BL/6 and HO hCCN4 knockin mice, as the antibody was cross-reactived between human and mouse CCN4. 

Abbr. HO, homozygous; WT, wild type; PC, positive control, Caco2 cells.