Acan-IRES-CreERT2

Nomenclature

C57BL/6Smoc-Acanem1(IRES-CreERT2)Smoc

Cat. NO.

NM-KI-200143

Strain State

Repository Live

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Gene Summary

Gene Symbol
Acan

Synonyms

Agc; cmd; Agc1; Cspg1; b2b183Clo

NCBI ID

11595

MGI ID

99602

Ensembl ID

ENSMUSG00000030607

Pubmed

Acan

Human Ortholog

ACAN

Model Description

A IRES-CreERT2 expression cassette was knocked into the Acan gene stop codon site via CRISPR/Cas9 mediated recombination.
Research Application：Cre recombinase tool; When crossed with mice carrying a targeted floxed alleles, this strain is useful in eliminating tissue-specific conditional expression of the gene. These mice could be used to study degenerative cartilage diseases such as osteoarthritis and degenerative disc disease.

Validation Data

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Fig. 1 CreERT2-mediated recombination in the joints of AcanCreERT2/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the growth plate and articular cartilage of AcanCreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

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Fig. 2 Detection of tdTomato(red) in various tissues of AcanCreERT2/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in the growth plate and articular cartilage after tamoxifen treatment. Tdtomato expression can be also detected in individual cells derived from the brown fat, spleen, testis, skin, skeletal muscle and cardiac valves. Tdtomato expression can not be detected in the brain, thymus, retina, lung, liver, stomach, submandibular gland, kidney, pancreas, intestine, colon, skin and myocardium. (For more detailed information please contact our technical advisor.)


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