Nkx2-5-IRES-Cre

Nomenclature

C57BL/6Smoc-Nkx2-5em1(IRES-Cre-WPRE-polyA)Smoc

Cat. NO.

NM-KI-200152

Strain State

Sperm cryopreservation

Export PDF

Model Description

A IRES-Cre-WPRE-polyA expression cassette was knocked into the Nkx2-5 gene stop codon site.
Research Application：Cre recombinase tool; Nkx2.5 acts as a key transcription factor and plays essential roles for heart formation. By mating the reporter mice with Cre-expressing mice, Nkx2-5 positive cells derived from double-positive mice were permanently labeled by fluorescent protein. When crossed with mice carrying a targeted floxed alleles, this strain is useful in eliminating tissue-specific conditional expression of the gene.

Validation Data

image.png

Fig. 1 Cre-mediated recombination in the heart of Nkx2-5Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the myocardial cells and vascular cells of Nkx2-5Cre/+; Rosa26tdTomato/+ mouse.

image.png

Fig. 2 Detection of tdTomato(red) in various tissues of Nkx2-5Cre/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in the heart, bronchus, pulmonary alveoli and submandibular gland. Tdtomato expression can be also detected in individual cells derived from the brain, retina, skin, kidney, pancreas, intestine, colon, spleen and thymus, except for pituitary and muscle. (For more detailed information please contact our technical advisor.)


You may also like

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

Learn more
Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

Learn more