hTNFR2(BALB/c)

Nomenclature

BALB/cAnSmoc-Tnfrsf1btm(hTNFRSF1B)Smoc

Cat. NO.

NM-HU-220499

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
TNFRSF1B

Synonyms

p75; TNFBR; Tnfr2; CD120b; TNF-R2; TNFR80; TNFRII; Tnfr-1; TNF-R75; TNF-R-II; TNF-alphaR2; TNFalpha-R2

NCBI ID

21938

MGI ID

1314883

Ensembl ID

ENSMUSG00000028599

Pubmed

TNFRSF1B

Human Ortholog

TNFRSF1B

Model Description

The endogenous mouse Tnfrsf1b gene was replaced by human TNFRSF1B gene.

Validation Data

image.png

Fig.1 Expression analysis of hTNFR2 on Treg cells in spleen of hTNFR2 humanized mice. 

Leukocytes were collected from spleen of wild-type balb/c mice (female, 8 weeks) and homozygous hTNFR2 mice (male, 14 weeks) stimulated in vivo with anti-CD3ε (50 µg/mouse, 24-hour stimulation, i.v.) and analyzed by flow cytometry with anti-mTNFR2 and anti-hTNFR2. hTNFR2 were exclusively detectable in hTNFR2 humanized mice but not in wild-type mice.

image.png

Fig.2 Expression analysis of hTNFR2 on DCs, monocyte, B cells, and neutrophils in spleen of hTNFR2 humanized mice. 

Leukocytes were collected from spleen of wild-type balb/c mice (female, 12 weeks) and homozygous hTNFR2 mice (male, 18 weeks), and analyzed by flow cytometry with anti-mTNFR2 and anti-hTNFR2. hTNFR2 were exclusively detectable in hTNFR2 humanized mice but not in wild-type mice.

image.png

Fig.3 Expression analysis of hTNFR2 on monocyte, B cells, and neutrophils in blood of hTNFR2 humanized mice. 

Leukocytes were collected from blood of wild-type balb/c mice (female, 12 weeks) and homozygous hTNFR2 mice (male, 18 weeks), and analyzed by flow cytometry with anti-mTNFR2 and anti-hTNFR2. hTNFR2 were exclusively detectable in hTNFR2 humanized mice but not in wild-type mice.

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