hRIPK1

Nomenclature

C57BL/6Smoc-Ripk1em1(hRIPK1)Smoc

Cat. NO.

NM-HU-233240

Strain State

Repository Live

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Gene Summary

Gene Symbol
Ripk1

Model Description

The endogenous mouse Ripk1 gene was replaced by human RIPK1 gene.

Validation Data

image.png

Fig.1 Detection of RIPK1 expression in the colon and lung of 7-week-old male mice by RT-PCR. 

Wild type: one band at 265 bp with primers F1/R1(mRipk1);

Heterozygous: one band at 265 bp with primers F1/R1(mRipk1) and one band at 255 bp with primers F2/R2(hRIPK1).

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

image.png

Fig.2 Detection of hRIPK1 and mRipk1 expression in colon (A) and lung (B) by qPCR. 

7-week-old male WT and hRIPK1 knockin mice received an intraperitoneal injection of saline or LPS (5 mg/kg) at 2 hours before sacrifice (n=2). 

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

image.png

Fig.3 Detection of human RIPK1 expression in hRIPK1 knockin mice by WB. 

Abbr. M, marker; WT, wild type; HE, heterozygous.


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