hCFTR

Nomenclature

C57BL/6Smoc-Cftrtm1(hCFTR)Smoc

Cat. NO.

NM-HU-225060

Strain State

Repository Live

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Gene Summary

Gene Symbol
Cftr

Model Description

The endogenous mouse Cftr gene was replaced by human CFTR gene.

Validation Data

image.png

Fig.1 Detection of CFTR expression in testis by RT-PCR. 

Wild type: only one band at 209 bp with primers F1/R1(mCftr);

Homozygous: only one band at 227 bp with primers F2/R2(hCFTR).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of human CFTR expression in hCFTR knockin mice by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous; PC, positive control, HepG2 cells.

Note. The anti-human CFTR antibody cross-reacted with mouse and humanized CFTR.



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