Pah-KO

Fig.1 Detection of Pah expression in liver and kidney of 16-week-old male WT and Pah-KO mice by RT-PCR. 

Wild type: one band at 193 bp with primers F1/R1(m Pah) and one band at 178 bp with primers F/R(mGapdh).

Homozygous: no band at 193 bp with primers F1/R1(m Pah) and one band at 178 bp with primers F/R(mGapdh).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of Pah mRNA expression in liver and kidney of 16-week-old male WT and Pah-KO mice by crop. 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.3 Detection of mouse PAH expression of 16-week-old male WT and Pah-KO mice by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous.

Fig.4 Characterization of WT C57BL/6 and Pah-KO mice. 

(A) The Pah-KO mice showed lower body weights than the WT mice (n=20/group). (B) Summary of blood Phe at various timepoints is shown (n=20/group). (C) Summary of blood Tyr at various timepoints is shown (n=20/group). Note: Data from the third party.

Fig.5 Hair coat color in WT C57BL/6 and Pah-KO mice. 

Hair coat of the Pah-KO mouse is light brown whereas it is dark brown for the WT C57BL/6 mouse.

Note: Data from the third party.

Fig.6 Brain weight analysis of 16-17-week-old WT and HO Pah-KO mice. (n=3,  Mean ± SEM. t-test, **p < 0.01).

Brain weight analysis showed that the brain weight of Pah-KO mice is significantly reduced.