hIL4/hIL4R(BALB/c)

Nomenclature

BALB/cJSmo-Il4tm3(hIL4)Il4ratm1(hIL4RA)Smoc

Cat. NO.

NM-HU-220167

Strain State

Embryo cryopreservation

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Model Description

The endogenous mouse Il4,Il4ra gene was replaced by human IL4 gene.

Validation Data

image.png

Fig.1 Detection of IL4 expression in thymus by RT-PCR. 

Wild type: only one band at 210 bp with primers F1/R1(mIl4);

Homozygous: only one band at 162 bp with primers F2/R2(hIL4).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of IL4R expression in thymus by RT-PCR. 

Wild type: only one band at 172 bp with primers F1/R1(mIl4ra);

Homozygous: only one band at 208 bp with primers F2/R2(hIL4R).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.


image.png

Fig.3 Detection of hIL4/hIL4RA (BALB/c) expression in serum by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. hIL4/hIL4RA (Balb/c) and BALB/c mice were i.p. injected with ConA for 6h.

image.png

Fig.4 Detection of hIL4-Ra and mIL4-Ra expression in spleen in WT BALB/c and HO/HO hIL4/hIL4R mice.

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Fig.5 Detection of hIL4-Ra and mIL4-Ra expression in spleen in WT BALB/c and HO/HO hIL4/hIL4R mice.

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