hIL21/hIL21R

Nomenclature

C57BL/6Smoc-Il21tm1(hIL21)Il21rtm1(hIL21R)Smoc

Cat. NO.

NM-HU-220038

Strain State

Embryo cryopreservation

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Model Description

hIL21(NM-HU-200255) were crossed with hIL21R(NM-HU-210031) to generate hIL21/hIL21R mice.

Validation Data

image.png

Fig 1. Detection of IL21 expression in splenocyte by RT-PCR. 

Wild type: only one band at 272 bp with primers F1/R1 (mIl21);

Homozygous: only one band at 304 bp with primers F2/R2 (hIL21); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig 2. Detection of hIL21 expression in culture supernatants of splenocyte by ELISA (n=3). 

Abbr. HO, homozygous; WT, wild type.

Note. Splenocytes from C57BL/6 and homozygous hIL21/hIL21R dKI mice were stimulated with anti-mCD3 antibody in vitro.

image.png

Fig 3. Detection of IL21R expression on spleen-derived activated T cells and B cells in hIL21/hIL21R dKI mice.

Note. Splenocytes from C57BL/6 and homozygous hIL21/hIL21R dKI mice were stimulated with anti-mCD3 antibody in vitro.

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