hIL11/hIL11RA

Nomenclature

C57BL/6JSmo-Il11tm1(hIL11)Il11ra1tm1(hIL11RA)Smoc

Cat. NO.

NM-HU-210372

Strain State

Repository Live

Export PDF

Model Description

hIL11(NM-HU-204981) was crossed with hIL11RA (NM-HU-204982) to generate hIL11/hIL11RA mice.
Research Application：inmmue therapy;drug screening

Validation Data

image.png

Fig.1 Detection of IL11 expression in testis,stomach and lung by RT-PCR in hIL11/hIL11RA dKI mice. 

Wild type: only one band at 171 bp with primers F1/R1(mIl11);

Homozygous: only one band at 162 bp with primers F2/R2(hIL11). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of IL11RA expression in testis, stomach and lung by RT-PCR in hIL11/hIL11RA dKI mice.  

Wild type: only one band at 289 bp with primers F1/R1(mIl11ra);

Homozygous: only one band at 247 bp with primers F2/R2(hIL11RA).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of hIL11 expression in lung homogenate by ELISA in hIL11/hIL11RA dKI mice. 

Abbr. HO, homozygous; WT, wild type. 

Note. hIL11/hIL11RA and C57BL/6 mice were injected with LPS.

image.png

Fig.4 Detection of human IL11RA expression in hIL11/hIL11RA dKI mice by WB. 

Abbr. HO, homozygous; WT, wild type.

Note. The IL11RA Antibody cross-reacted with mouse and humanized IL11RA. 

image.png

Fig.5 Detection of hIL11RA expression in Kidney in C57BL/6 and HO/HO hIL11/hIL11RA knockin mice by IHC. 

Abbr. HO/HO, homozygous; WT, wild type. 

Note. The IL11RA Antibody cross-reacted with mouse and humanized IL11RA. 

You may also like

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

Learn more

【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

Learn more

Cre-lox系统介绍及使用汇总

你一定听说过Cre-lox重组系统，无论你是否直接进行过基因操作。由于Cre-lox系统具有操作简单、重组率高的优点，如今已经成为体内外遗传操作的强有力工具。利用Cre-lox系统，可以在特定细胞、组织或整个生物体，甚至在特定时间点敲除或表达某个基因，实现对特定基因的时空特异性操作，这对基因功能的研究和人类疾病动物模型的建立都具有深刻影响。

Learn more