hIL2

Nomenclature

C57BL/6Smoc-Il2tm3(hIL2)Smoc

Cat. NO.

NM-HU-190048

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
IL2

Synonyms

Il-2

NCBI ID

16183

MGI ID

96548

Ensembl ID

ENSMUSG00000027720 

Pubmed

IL2

Human Ortholog

IL2

Model Description

The endogenous mouse Il2 gene was replaced by human IL2 gene.
Research Application：Immunotherapy,cancer research,drug screening

Validation Data

image.png

Fig1. Detection of IL2 expression by RT-PCR. 

Wild type: only one band at 255 bp with primers F2/R2(mIl2); Homozygous: only one band at 320 bp with primers F1/R1(hIL2). 

Abbr. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

IL2-Hu.png

Fig2. Expression of human IL2 in the Serum of IL2-HU heterozygous mice is detected by ELISA.

IL2-Mu.png


Fig3. Expression of murine IL2 in the Serum of IL2-HU heterozygous mice and  is detected by ELISA.

IL2-3.png

Fig4. Splenocytes from humanized IL2 mice were analyzed by flow cytometry after activation. 

hIL2+ (but not mIL2+) cells were detectable in the homozygous hPD-L2 mice. 

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