hFGFR3-G380R

Fig.1 Detection of FGFR3 expression in knee joint and brain in 2.4-week-old male and female hFGFR3-G380R knockin mice by RT-PCR. 

HepG2 cells were used as the WT control of human FGFR3 RNA. 

Wild type: one band at 217 bp with primers F1/R1(mFgfr3) and one band at 123 bp with primers F/R(mGapdh);

Heterozygous: one band at 217 bp with primers F1/R1(mFgfr3), one band at 375 bp with primers F2/R2(hFGFR3) and one band at 123 bp with primers F/R(mGapdh). 

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

Fig.2 Sequencing analysis of human FGFR3 mRNA in HepG2 cells and hFGFR3-G380R knockin mice. 

Sequencing data of RT-PCR products confirmed the presence of a G to A mutation in human FGFR3 mRNA expressed in hFGFR3-G380R knockin mice. 

Abbr. HE, heterozygous.

Fig.3 Body weight of WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice. (n=1)

Fig.4 Micro-CT analysis of skeleton in 35-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

Fig.5 Micro-CT analysis of skeleton in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

Fig.6 Micro-CT analysis of skeleton in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

Fig.7 Micro-CT analysis of skeleton in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).

Fig.8 Micro-CT analysis of femur in 35-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

Fig.9 Micro-CT analysis of femur in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

Fig.10 Micro-CT analysis of femur in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R mice.

Fig.11 Micro-CT analysis of femur in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).

Fig.12 Micro-CT analysis of skull in 35-day-old  WT C57BL/6 and heterozygous hFGFR3-G380R mice.

Fig.13 Micro-CT analysis of skull in 49-day-old WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice.

Fig.14 Micro-CT analysis of skull in 63-day-old WT C57BL/6 and heterozygous hFGFR3-G380R knockin mice.

Fig.15 Micro-CT analysis of skull in WT C57BL/6 and heterozygous hFGFR3-G380R mice (n=1).