hCA4

Nomenclature

C57BL/6Smoc-Car4tm1(hCA4)Smoc

Cat. NO.

NM-HU-234802

Strain State

Repository Live

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Gene Summary

Gene Symbol
Car4

Model Description

The endogenous mouse Car4 gene was replaced by human CA4 gene.

Validation Data

image.png

Fig.1 Detection of CA4 expression in colon by RT-PCR. 

Wild type: only one band at 115 bp with primers F1/R1(mCar4);

Homozygous: only one band at 136 bp with primers F2/R2(hCA4).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of human CA4 expression in hCA4 mice by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous; PC, positive control, MCF-7 cells.

Note. The anti-human CA4 antibody cross-reacted with mouse CA4 and humanized CA4.


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