hIFNAR1/hIFNAR2

Nomenclature

C57BL/6Smoc-Ifnar1tm1(hIFNAR1)Ifnar2tm1(hIFNAR2)Smoc

Cat. NO.

NM-HU-234770

Strain State

Repository Live

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Gene Summary

Gene Symbol
Ifnar2

Synonyms

Ifnar-2; AI747302

NCBI ID

15976

MGI ID

1098243

Ensembl ID

ENSMUSG00000022971

Pubmed

Ifnar2

Human Ortholog

IFNAR2

Model Description

On the basis of strain hIFNAR1(NM-HU-220355), the endogenous mouse Ifnar2 gene was replaced by human IFNAR2 gene.

Validation Data

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Fig.1 Detection of IFNAR1 expression in thymus and subcutaneous fat pad by RT-PCR. 

Wild type: only one band at 262 bp with primers F1/R1(mIfnar1);

Homozygous:  only one band at 471 bp with primers F2/R2(hIFNAR1).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of human IFNAR1 expression in the male C57BL/6 WT mouse (8-10 weeks old) and hIFNAR1 knockin mice (12 weeks old) by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous; PC, positive control, Hela cells.

image.png

Fig.3 Detection of human IFNAR2 expression in the male C57BL/6 WT mouse (8-10 weeks old) and hIFNAR1/hIFNAR2 knockin mice (12 weeks old) by FCM. 

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Fig.4 Detection of pStat1 expression in CD4+ T cells in the C57BL/6 WT mouse (8-10 weeks old) and hIFNAR1/hIFNAR2 knockin mice (20 weeks old) by FCM. 

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Fig.5 Detection of pStat1 expression in CD4+ T cells in the C57BL/6 WT mouse (8-10 weeks old) and hIFNAR1/hIFNAR2 knockin mice (20 weeks old) by FCM. 

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Fig.6 Detection of pStat1 expression in CD8+ T cells in the C57BL/6 WT mouse (8-10 weeks old) and hIFNAR1/hIFNAR2 knockin mice (20 weeks old) by FCM. 

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Fig.7 Detection of pStat1 expression in CD8+ T cells in the C57BL/6 WT mouse (8-10 weeks old) and hIFNAR1/hIFNAR2 knockin mice (20 weeks old) by FCM. 

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