hRIPK1

Fig.1 Detection of RIPK1 expression in the colon and lung of 7-week-old male mice by RT-PCR. 

Wild type: one band at 265 bp with primers F1/R1(mRipk1);

Heterozygous: one band at 265 bp with primers F1/R1(mRipk1) and one band at 255 bp with primers F2/R2(hRIPK1).

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

Fig.2 Detection of hRIPK1 and mRipk1 expression in colon (A) and lung (B) by qPCR. 

7-week-old male WT and hRIPK1 knockin mice received an intraperitoneal injection of saline or LPS (5 mg/kg) at 2 hours before sacrifice (n=2). 

Abbr. M, DNA marker; HE, heterozygous; WT, wild type.

Fig.3 Detection of human RIPK1 expression in hRIPK1 knockin mice by WB. 

Abbr. M, marker; WT, wild type; HE, heterozygous.