hMAPT-P301S

Fig.1 Detection of hMAPT-P301S expression in brain by RT-PCR. (male, 7wks)

Wild type: only one band at 245 bp with primers F1/R1 (mMapt);

Heterozygous: one band at 245 bp with primers F1/R1 (hMAPT).

Abbr. M, DNA marker;  HE, heterozygous; WT, wild type.

Fig.2 Sequencing results of the MAPT locus in the wild type and heterozygous hMAPT-P301S mice.

Fig.3 Detection of Phospho-Tau expression in brain in hMAPT-P301S mice by WB. (female, 24wks)

The relative gray values to GAPDH were quantitated by densitometry using ImageJ software.

Abbr. M, marker; WT, wild type; HO, homozygous.

Note. UnPaired t-test, *P < 0.01.