hC5AR1/C5AR2

Nomenclature

C57BL/6JSmo-C5ar1tm1(hC5AR1)C5ar2tm1(hC5AR2)/Smoc

Cat. NO.

NM-HU-2000018

Strain State

Embryo cryopreservation

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Model Description

The endogenous mouse C5ar1 and C5ar2 gene was replaced by human C5AR1 and hC5AR2 gene.
Research Application：Immunotherapy,drug screening

Validation Data

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Fig1. Detection of C5AR1 and C5AR2 expression in spleen by RT-PCR. 

Wild type: only one band at 257 bp with primers F1/R1 (mC5ar1), only one band at 506 bp with primers F3/R3 (mC5ar2);

Homozygous: only one band at 313 bp with primers F2/R2 (hC5AR1),  only one band at 318 bp with primers F4/R4 (hC5AR2).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.image.png

Fig2. Detection of C5AR1 expression on myeloid cells in blood in hC5AR1 knockin mice.

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Fig3. Detection of C5AR1 expression on myeloid cells in bone morrow in hC5AR1 knockin mice.

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Fig4. Detection of C5AR2 expression on myeloid cells in spleen in hC5AR1/hC5AR2 dKI mice. (Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)

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Fig5. Detection of C5AR2 expression on myeloid cells in bone marrow in hC5AR1/hC5AR2 dKI mice. (Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)

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Fig6. Detection of hC5AR2 expression on myeloid cells in peritoneal lavage fluids in hC5AR1/hC5AR2 dKI mice. (Note: Anti-human C5L2 (C5AR2) antibody was cross-binding with mouse C5AR2)

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