Tnf-KO

Nomenclature

C57BL/6Smoc-Tnfem1Smoc

Cat. NO.

NM-KO-00100

Strain State

Repository Live

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Gene Summary

Gene Symbol
Tnf

Synonyms

DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha

NCBI ID

21926

MGI ID

104798

Ensembl ID

ENSMUSG00000024401

Pubmed

Tnf

Human Ortholog

TNF

Model Description

Exon2-3 of Tnf gene were targeted to generate the Tnf knockout mice.

Research Application：Immune and cancer research

Disease Connection

Rheumatoid Arthritis

Phenotype(s)

MGI:3622060

Reference(s)

Kontoyiannis D, Pasparakis M, Pizarro TT, Cominelli F, Kollias G, Impaired on/off regulation of TNF biosynthesis in mice lacking TNF AU- rich elements: implications for joint and gut-associated immunopathologies. Immunity. 1999 Mar;10(3):387-98

rheumatoid arthritis

Phenotype(s)

MGI:3622062

Reference(s)

Kontoyiannis D, Pasparakis M, Pizarro TT, Cominelli F, Kollias G, Impaired on/off regulation of TNF biosynthesis in mice lacking TNF AU- rich elements: implications for joint and gut-associated immunopathologies. Immunity. 1999 Mar;10(3):387-98

Spondyloarthropathy

Phenotype(s)

MGI:3629514

Reference(s)

Sakkou M, Chouvardas P, Ntari L, Prados A, Moreth K, Fuchs H, Gailus-Durner V, Hrabe de Angelis M, Denis MC, Karagianni N, Kollias G, Mesenchymal TNFR2 promotes the development of polyarthritis and comorbid heart valve stenosis. JCI Insight. 2018 Apr 5;3(7)

Validation Data

image.png

Fig.1 Detection of TNF expression in spleen and thymus by RT-PCR. 

Wild type: only one band at 209 bp with primers F1/R1(mTnf). 

Abbr. M, DNA marker;  HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of mTnf expression in spleen and thymus by Q-PCR. 

image.png

Fig.3 Detection of mTNF-a expression in serum by ELISA (male, 8wks, n=2). 

Abbr. HO, Homozygous; WT, wild type.

Note.TNF-KO and C57BL/6 mice were i.p. injected with LPS.

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